Figure 2.

Deep profiling of circulating Spike- specific B cells by flow cytometry in patients. (A) Gating strategy for identification of Spike- specific B cells. Spike⁺ B cell gate was set based on the background level binding of CD3⁺ T cells. (B) Frequencies (left) and absolute numbers (right) of Spike- specific B cells in patients with disease or in early and late convalescence. Each dot represents one biologically individual sample taken from one patient. n(disease)=24; n(Early conv.)=32; n(Late conv.)=25. (C) Longitudinal analysis of frequencies (left) and absolute numbers (right) of Spike- specific B cells in patients 3-7 months after the disease. Each connected dot represent one individual patient (n=11). (D) Phenotype comparison of endogenous naïve and memory B cells and Spike- specific B cells. Shown is one representative sample from a patient in late convalescence. Statistics: Kruskal-Wallis multiple comparison test with Dunn’s post hoc correction. *p<0.05.