FIG. 2.

C. neoformans expresses [³H]CsA binding activities. CsA-binding protein activity was measured by the LH-20 drug binding assay. (A) Tritiated CsA alone (squares) or incubated with purified S. cerevisiae cyclophilin A protein (diamonds) was subjected to Sephadex LH-20 chromatography. Fractions were analyzed by scintillation counting, and the percentages of total counts per minute were plotted. (B) Protein extracts from the C. neoformans wild-type CPA1 CPA2 strain H99 (squares), cpa1 mutant strain PW67 (diamonds), cpa2 mutant strain PW71 (circles), and cpa1 cpa2 mutant strain PW62 (triangles) were subjected to Sephadex LH-20 chromatography. Fractions were analyzed by scintillation counting, and the percentages of total counts per minute were plotted. Elution volume is given in milliliters.