Figure 5.

Immunodetection of EhVps23 in Neo-EhVps23 and Neo trophozoites and in the secreted products by trophozoites. (A) Confocal images of trophozoites and their secreted products treated with α-EhVps23, α-EhADH, α-EhCP112 antibodies and fluoresceinated secondary antibodies, according to the specie of each one of the antibodies used. Scale bar = 10 μm. (B) Ponceau stain and western blot of trophozoites extracts (E) and their secretion products (S) separated by SDS-PAGE and blotted with α-EhVps23, α-HA, α-EhVps32, α-EhADH, and α-Ubiquitin. α-EhCP112 was used as a positive control and α-actin as a control of the absence of trophozoites debris in the supernatants.