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. 2022 Mar 14;12:835654. doi: 10.3389/fcimb.2022.835654

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Copyright © 2022 Galindo, Javier-Reyna, García-Rivera, Bañuelos, Chávez-Munguía, Salazar-Villatoro and Orozco

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron microscopy of vesicles found in secretion products. (A–D) Transmission electron microscopy of negative stained vesicles in the secretion products. The numbers indicate the different sizes calculated by the transmission electron microscope program JEM-1011. (E, F) TEM images (15 000X) of purified extracellular vesicles, the thin sections were incubated with α-EhVps23 followed by gold-labelled α-rat antibodies (10 nm). Squared areas are magnified to show EhVps23 protein in vesicles of different sizes. Arrowheads: label of the antibodies used to detect EhVps23. Scale: 200 nm.