Figure 9.

Motility of Neo-EhVps23 and Ehvps23-KD trophozoites. The trophozoites were placed in the upper chamber of Transwell filters and bovine serum in the lower chamber. After 3 h the trophozoites in the lower chamber was counted. (A) Neo-EhVps23 trophozoites found in the lower chamber of the Transwell filters. As control we used Neo trophozoites. (B) Quantification of trophozoites in the lower chamber to obtain the relative motility of Neo-EhVps23 trophozoites with respect to the control. (C, D) Western blot of silenced trophozoites (Ehvps23-KD) probed with the α-EhVps23 antibodies. Densitometry at the left using α-actin as control. (E) Ehvps23-KD trophozoites found in the lower chamber of the Transwell filters. As control we used trophozoites of the wild type strain HM1:IMSS. (F) Quantification of trophozoites in the lower chamber to obtain the relative motility of Ehvps23-KD trophozoites with respect to the control. **P < 0.01, ***P < 0.001.