FIGURE 4.
PF induces SOCS3 expression to inhibit the expression of p-ASK1 and TF in RAW264.7 cells. (A) RAW264.7 cells were treated with different doses of PF (10−8, 10−7, 10−6, 10−5, and 10−4 M) for 4 h, and the expression of SOCS3 was determined by western blotting. (B,C) Different doses of PF were pre-administered for 4 h, and LPS (1 μg/ml) was then administered for 12 h; the expressions of p-ASK1, ASK1, and TF were determined by western blotting. (D) ASK1 inhibitor NQDI-1 (600 nM) was pre-administered for 0.5 h, and LPS (1 μg/ml) was then administered for 12 h; the expression of TF was determined by western blotting. (E) The expression of SOCS3 after PF (10−6 M) administration with or without LPS (1 μg/ml). n = 3 per group; *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control group; ## p < 0.01 and ### p < 0.001 vs. LPS group.