FIGURE 6.

PF promotes SOCS3 expression via activating Gas6/Axl axis. The expression of p-Axl was determined by western blotting in RAW264.7 cells (A) and human umbilical vein endothelial cells (HUVEC) (D). The level of Gas6 protein and mRNA was measured in the supernatant of RAW264.7 cells (B,C) and HUVEC (E,F). RAW264.7 and HUVEC were administered with PF (10⁻⁶ M) for 4 h. The expressions of p-Axl (G) and Gas6 (H) after PF (10⁻⁶ M) administration with or without LPS (1 μg/ml) in Raw264.7 cells. (I) The expression of SOCS3, p-ASK1, and ASK1 was measured in Raw264.7 cells after Axl knockdown. n = 3 per group; *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control group; ^# p < 0.05, ^## p < 0.01, and ^### p < 0.001 vs. LPS group; ^$$ p < 0.01 vs. LPS + PF group.