Table 2.
Results of analysis of variance: significance (P) of the sources of variation affecting sperm viability and CASA parameters in experiment 1.
| Sperm variables1 | Sources of variation2 | ||
|---|---|---|---|
| pH | ET | pH∗ET | |
| SMI (%) | ns | ns | ns |
| TMS (%) | 0.0004 | 0.0196 | ns |
| PMS (%) | 0.0096 | ns | ns |
| VCL (μm/s) | 0.0003 | 0.0175 | ns |
| VSL (μm/s) | 0.0001 | 0.0001 | ns |
| VAP (μm/s) | 0.0001 | 0.0004 | ns |
| LIN (%) | 0.0003 | 0.0001 | ns |
| STR (%) | ns | 0.0001 | ns |
| WOB (%) | 0.0001 | 0.0003 | ns |
| ALH (μm) | 0.0007 | 0.0001 | ns |
| BCF (Hz) | 0.0203 | 0.0001 | ns |
1SMI: sperm membrane integrity; TMS: total motile sperm; PMS: progressive motile sperm; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN = VSL/VCL × 100; STR = VSL/VAP × 100; WOB = VAP/VCL × 100; ALH: amplitude of lateral head displacement; BCF: beat cross frequency. 2pH = pH of Lake extender; ET: equilibration temperature after semen dilution.