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. 2022 Mar 14;13:800618. doi: 10.3389/fmicb.2022.800618

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Copyright © 2022 Qu, Li, Li, Liao and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T cell population of clusters 6 and 7 were further divided into four distinct cell populations. (A) UMAP display of the cell populations and their proportions of the T cell sub clustering (clusters 6 and 7) in the control PBMCs. (B) UMAP display of the cell populations and their proportions of the T cell sub clustering (clusters 6 and 7) in PBMCs from ALV-J infected chicken. (C) The regrouped T cell distribution and proportion of each cluster in PBMCs from ALV-J infected and control chickens. (D) Top five DEGs (x-axis) identified in clusters A0–A3 (y-axis). (E) Expression levels of characteristic marker genes (CD3D, CD28, and CD8A) in clusters A0–A3.