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. 2022 Mar 14;13:800618. doi: 10.3389/fmicb.2022.800618

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Copyright © 2022 Qu, Li, Li, Liao and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pseudotime analysis of clusters A0, A1, and A2. (A) Mapping of clusters A0 (Th0), A1 (Th2-like), and A2 (Thl-like) to the pseudotime trajectory. (B) Pseudotime trajectory calculated from all cells of clusters A0–A2 in the control and ALV-J infected samples. Darker colored dots represent a shorter pseudotime and earlier differentiation period. (C) Mapping of cells in control and ALV-J infected samples to the pseudotime trajectory. (D) The cell states of pseudotime trajectory partitioned from all cells of clusters A0–A2 in the control and ALV-J infected samples. (E) Cell proportion of each state between the ALV-J infected and control samples. (F) Dynamics of the top 10 branching DEGs. Full line: state 1, 2; imaginary line: state 1, 3.