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. 2022 Mar 14;9:799703. doi: 10.3389/fmolb.2022.799703

FIGURE 2.

FIGURE 2

Inhibition of HMOs on the binding between HepG2 cells and Spike protein RBD. Fluorescent labelled proteins were preincubated with 1 mg per ml 2′-FL, 6′-SL, and LNnT, respectively. The preincubation was performed at room temperature for 30 min. (A) Immunofluorescence for S protein RBD binding. The columns (from left to right) show staining of nuclear acid (Hoechst 33342), plasma membrane (CellMask™ Deep Red), S protein RBD, and merged image. Scale bar, 496 pixels. (B) Quantification of fluorescent intensity of spike protein RBD binding. Fluorescence intensity was quantified for selected cell area. Quantification was performed with software ImageJ. Asterisks indicate the statistical significance between groups compared (**p< 0.01%; ***p< 0.001%; ns p < 0.05).