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. 2022 Mar 14;9:799703. doi: 10.3389/fmolb.2022.799703

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Copyright © 2022 Sheng, Vinjamuri, Alvarez, Xie, McGrath, Chen, Barboza, Frieman and Lebrilla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Host Cell Surface Glycome Modification. 2F-Fucose (Fucosyltransferase Inhibitor); 3-F-Sia (Sialyltransferase Inhibitor). (A) Metabolic engineering stargey for altering host cell glycosulation. Symbol nomenclature is used for representing glycan structures (https://www.ncbi.nlm.nih.gov/glycans/snfg.html). (B) N-Glycome Profiles of unmodified and modified HepG2 cells from LC-MS analysis. Compound list and details are shown in Supplementary Table S3. Pie charts were color coded to assign glycan subtype. Numbers inside pie charts denote the relative abundance of each identified glycan subtype.