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. 2022 Mar 14;9:799703. doi: 10.3389/fmolb.2022.799703

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Copyright © 2022 Sheng, Vinjamuri, Alvarez, Xie, McGrath, Chen, Barboza, Frieman and Lebrilla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sialylated HMOs diminished binding of spike protein RBD to HepG2 Cells. Fluorescent labelled proteins were preincubated with 1 mg per ml 3′-FL (3′-sialyllactose) and 6′-SL (6′-sialyllactose) respectively at room temperature. (A) Immunofluorescence for S protein RBD binding with cells. The columns (from left to right) show staining of nuclear acid (Hoechst 33342), plasma membrane (CellMask™ Deep Red), S protein RBD, and merged image. Scale bar, 600 pixels. (B) Quantification of fluorescent intensity of spike protein RBD binding. Fluorescence intensity was quantified for selected cell area. Quantification was performed with software ImageJ. Asterisks indicate the statistical significance between groups compared (*p< 0.05%; ns p < 0.05).