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. 2022 Mar 14;13:765317. doi: 10.3389/fmicb.2022.765317

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Copyright © 2022 Taki, Watanabe, Sato’o, Tan, Ono, Kiga, Aiba, Sasahara, Azam, Thitiananpakorn, Veeranarayanan, Li, Zhang, Kawaguchi, Hossain, Maniruzzaman, Hu and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of titer of toxic shock syndrome toxin-1 (TSST-1) produced in 157 TSST-1-producing clinical isolates of Staphylococcus aureus cultured in (A) Todd Hewitt (TH) medium or (B) TH medium supplemented with horse serum. The titer of TSST-1 was determined by using the passive latex agglutination kit. The results of a single representative experiment carried out with three technical replicates are shown. Note that unimodal distribution of TSST-1 titers turned into bimodal distribution when the strains were cultured in the medium supplemented with horse serum.