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. 2022 Mar 14;13:765317. doi: 10.3389/fmicb.2022.765317

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Copyright © 2022 Taki, Watanabe, Sato’o, Tan, Ono, Kiga, Aiba, Sasahara, Azam, Thitiananpakorn, Veeranarayanan, Li, Zhang, Kawaguchi, Hossain, Maniruzzaman, Hu and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Comparisons of tst-carrying Staphylococcus pathogenicity islands (SaPIs). The SaPI loci were extracted from the whole-genome sequence of each S. aureus strain included in Figures 2, 3 with the exception of JMUB3035 due to the lack of complete genome sequence and aligned for comparison. Strains isolated from non-mTSS patients are underlined. Note that the overall gene localizations, including the location of the tst gene, are well conserved among strains. (B) Alignment of the DNA sequences of the tst promoter regions. The bent arrow indicates the transcription start site of the tst transcript, as determined in the previous study (Andrey et al., 2010) (designated position +1). The −10 and −35 regions are indicated by numeral numbers, and the SarA-binding site and putative second SarA-binding site are shown in the box and shadowed box, respectively. The variable T repeat region is highlighted in white characters.