Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 14;13:765317. doi: 10.3389/fmicb.2022.765317

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Taki, Watanabe, Sato’o, Tan, Ono, Kiga, Aiba, Sasahara, Azam, Thitiananpakorn, Veeranarayanan, Li, Zhang, Kawaguchi, Hossain, Maniruzzaman, Hu and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Comparison of promoter activity among the eGFP overexpression mutants carrying tst promoter with different number of T repeats (–six to nine) in the putative second SarA-binding site. The relative fluorescence intensities are shown as means ± standard deviation. The experiment was carried out in triplicate. (B) Comparison of serum-induced TSST-1 production between S. aureus mutants carrying 8- and 9-T repeats in the putative second SarA-binding site of tst promoter. TSST-1 productions were measured by ELISA and shown as means ± standard deviation. The experiment was carried out five times. The level of significance, calculated by Student’s t-test, is denoted by asterisks. (C) Comparison of the activities of tst promoters with 8-T and 9-T repeats between wild-type and sarA-knockout N315 strain. The relative fluorescence intensities are shown as means ± standard deviation. The experiment was carried out in both technical duplicate and biological quintuplicate (*, **, and *** indicate p-values < 0.05, 0.01, and 0.001, respectively).