Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 28;12:5291. doi: 10.1038/s41598-022-09364-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Live-cell imaging of Rac1 biosensors. Representative image series of a HeLa cell co-expressing the FP Rac1 sensor with the 20 nm ER/K linker (transient transfection) and RapR-Src constructs (stable transfection) was imaged at two-minute intervals. The first image obtained following rapamycin addition (500 nM) is indicated by the square (at t = 0). The montage shows FRET/mCerulean ratio images with warmer colors reflecting higher localized Rac1 activity.