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. 2022 Mar 29;13:1663. doi: 10.1038/s41467-022-29321-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Scheme for the stand-by single-molecule assay. Termination is mediated only by the stand-by ρ pre-bound on RNAP in the absence of catch-up ρ. b ρ-dependency of stand-by decomposing termination. The ρ-dependent TEs of decomposing (open) and recycling (solid) in the y-axis are plotted against ρ concentrations on a base-10 log scale in the x-axis. Recycling is virtually null over the entire range of ρ concentrations used, so the decomposing data overlap with their sum data (not shown). c Relative frequencies of readthrough (n = 326 complexes) and decomposing (n = 93) timings measured in stand-by assays. The data are fitted to single exponential functions. Error bar represents the standard deviation of the mean from n ≥ 3 independent experiments. The numbers of analyzed molecules for (b) are in Supplementary Table 2. Source Data file includes the data for (b, c).