Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 29;13:1663. doi: 10.1038/s41467-022-29321-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — For RNAP displacing tests, comparisons were made among 15 DNA templates with a 3-bp mismatch. ρ-dependent TEs (= raw TE—background TE) of recycling (solid) and decomposing (open) were measured in stand-by (red) and catch-up (cyan) assays and normalized to the maximum of 100% by dividing with (1—background TE). a Putative elongation-coupled progression of transcription bubble in the mgtA wild-type template and a mutant with a 3-bp mismatch (blue). b A peaked effect of the 3-bp mismatch on decomposing termination. Normalized decomposing TEs of the 15 mutants in the y-axis are plotted against the center position of the 3-bp mismatch in the x-axis. Horizontal lines indicate the values of the wild-type template (16.7% for stand-by assay and 24.9% for catch-up assay). c No effect of the mismatch on recycling termination. Normalized recycling TEs in the y-axis are plotted against the mismatch position in the x-axis. Error bar represents the standard deviation of the mean from n ≥ 3 independent experiments. The numbers of analyzed molecules for (b) and (c) are in Supplementary Table 2. Source Data file includes the data for (b) and (c).