Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 29;5:282. doi: 10.1038/s42003-022-03237-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schematic diagram showing treatment of astrocytes with control or activated microglia conditioned medium (MCM) b Double immunofluorescence of complement component 3 (C3, red) and astrocytes (GFAP, green) treated with control or activated MCM in primary astrocyte cultures. C3 and GFAP immunoreactivity overlay (merge) c Quantification of C3⁺ area (%) of control or activated MCM treated astrocytes. d Schematic diagram showing treatment of astrocytes with control or activated endothelial conditioned medium (ECM) e Double immunofluorescence of complement component 3 (C3, red) and astrocytes (GFAP, green) treated with control or activated ECM in primary astrocyte cultures. C3 and GFAP immunoreactivity overlay (merge) f Quantification of C3⁺ area (%) of control or activated ECM treated astrocytes. The number of cells analyzed was 20 per image from 10 different images per culture from 4 independent cultures. Results are shown as the median ± IQR of n = 4. Asterisks indicate significant differences, where *p < 0.05 by Mann-Whitney test. Scale bar 50 μm.