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. 2022 Mar 29;5:282. doi: 10.1038/s42003-022-03237-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Triple immunofluorescence of amyloid (Thio-S, green), astrocytes (GFAP, cyan), and Decorin (red) in the cortex of 15-month-old Tg-FDD, APP/PS1 mice and WT controls. Major presence of Decorin immunoreactivity is observed in the perivascular region of Thio-S positive vasculature in Tg-FDD mice. Yellow arrows indicate Decorin⁺, GFAP⁺ cells and purple arrows indicate Decorin-, GFAP⁺ cells. Colocalization analysis (CC) was performed to determine pixel intensity correlation between GFAP and Decorin. White pixels indicate colocalization between GFAP and Decorin signal and white dashed arrows represent distance in plot profiles showing overlapping intensities of astrocytes (GFAP, cyan) and Decorin (red). b Quantification of the proportion of Decorin⁺ cells in Tg-FDD, APP/PS1 and WT mice. c Triple immunofluorescence of astrocytes (GFAP, green), complement component 3 (C3, cyan), and Decorin (red) in the cortex of 15-month-old Tg-FDD and APP/PS1 mice. Yellow arrows indicate C3⁺, Decorin⁺, GFAP⁺ cells and purple arrows indicate C3⁺, Decorin-, GFAP⁺ cells. d Quantification of the proportion of C3⁺Decorin⁺ astrocytes versus C3⁺ astrocytes in Tg-FDD, APP/PS1 and WT mice. For quantifications, eight to ten images were used for each experiment (n = 3), and 200 cells were counted. All are representative images of 15-month-old Tg-FDD, APP/PS1 or WT mice. Scale bar 25 or 50 μm, respectively.