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. 2022 Mar 29;13(3):279. doi: 10.1038/s41419-022-04725-9

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Overlapped Acox2-interacting proteins in MHCC97H and HEK293T cells by TAP-MS analysis were displayed. B Anti-Myc immunoprecipitation assays were performed in HEK293T cells overexpressing Flag-tagged ACOX2 and Myc-tagged MCCC2 constructs followed by immunoblotting with the indicated antibodies. Specificity of antibodies was evaluated as indicated (left panel). MCCC2 methylcrotonoyl-CoA carboxylase subunit 2, MS mass spectrometry, TAP tandem affinity purification.