Skip to main content
. 2022 Mar 29;13(3):279. doi: 10.1038/s41419-022-04725-9

Fig. 8. Non-histone Kcr changes caused by Acox2 knockout might be involved in DNA damage responses.

Fig. 8

A Immunofluorescence staining with anti-gammaH2AX antibody in cells expressing Ehhadh WT or (K-R) mutants. B Immunoblot analysis of gammaH2AX and pan- or phospho-ATM and CHK2 in cells expressing Ehhadh WT or (K-R) mutants. Beta-actin served as a loading control. C, D Top3b protein levels were significantly downregulated in the livers of Acox2−/− mice by western Blot (C). Quantification data of Top3b expression level when normalized against Beta-actin (D). **p value < 0.01 (two-tailed Student’s t test; n = 6). A.U arbitrary units. Representative image from three independent experiments was displayed.