Fig. 3. Comparative effect of APOE3/3 versus APOE4/4 astrocyte conditioned media (ACM) on the lipid profile of wild-type (WT) neurons.
a Heatmap of lipids significantly altered in WT neurons after treatment with ACM derived from immortalized APOE3/3 and APOE4/4 astrocytes. Results expressed as Z-score [(average mol% of individual lipid species per genotype−average mol% of lipid in all genotypes)/standard deviation of average mol% lipid] represented in gradient color; blue and red indicate negative and positive Z-score, respectively. One-way ANOVA was performed and results threshold by p < 0.05; n = 6. b Volcano plots of differentially modulated lipid species under same conditions as in (a). Colored plots denote significant alterations (Tukey’s post-hoc p < 0.05), black plots denote unaltered species (p > 0.05). c–h Normalized average mol% (fold-change) of (c) CE, (d) TAG, (e) DAG, (f) PA (g) PC, (h) BMP lipid species from WT neurons incubated with ACM. Lipids were annotated per total acyl carbons and degree of unsaturation. Data represent median ± max/min. One-way ANOVA followed by Tukey’s post-hoc test for multiple comparisons was performed. *p < 0.05, **p < 0.01, and ***p < 0.001.