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. 2022 Mar 29;12:5341. doi: 10.1038/s41598-022-09428-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of fat drop. (A) Representative images of oil red O staining. Scale bar, 200 μm. (B) Quantification of oil red O staining. Lipotoxicity and glucolipotoxicity groups showed significantly more oil red O accumulation than control and glucotoxicity groups, n = 6, 3 independent experiments, **p < 0.01. (C) Representative images of fat drops (Osmium method). Red arrow indicates fat drop. Scale bar, 100 μm. (D) Representative images of TEM. Fat accumulation was observed in the glucolipotoxicity group. Scale bar, 10 μm. N: nuclear, FD: fat drops.