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. 2022 Mar 29;13:1656. doi: 10.1038/s41467-022-29315-3

Fig. 8. ABA and GA regulate ERF55 and ERF58 expression.

Fig. 8

a–d RT-qPCR analysis of ERF58 (a, b) and ERF55 expression (c, d) in seeds incubated in the dark for 12 h on different concentrations of ABA (a, c) or GA3 (b, d). ACT1 was used as an internal control. Values show means of three replicates ±SD. Different letters indicate significant differences as determined by one-way ANOVA followed by post-hoc Tukey’s HSD test; p < 0.05. e, f Effect of ABA and GA3 on HA-YFP-ERF58 protein levels in ERF58ox seeds. Col-0 p35S:HA-YFP-ERF58 (line 21C) seeds were incubated in the dark on 0.5 μM ABA (e) or 100 μM GA3 (f) for 1, 3, 6, or 12 h. Control seeds were incubated on water. Total protein was extracted and analysed by SDS-PAGE and immunoblotting with α-HA; α-ACT was used to detect ACTIN as loading control. Col-0 was included as negative control. Signals detected by α-HA and α-ACT were quantified using ImageJ; numbers below the membrane show the HA/ACT ratio. The experiments were repeated three times with similar results.