Table 2.

Prioritized serine hydrolase targets of AA692 identified by competitive activity-based protein profiling. See also Figure S4 and Table S1. Summary of ABPP-SILAC experiments, related to Table 2, Table S2. Peptides detected in ABPP-SILAC experiments, related to Table 2, Table S3. Active serine hydrolase proteome, related to Table 2, Table S4. Prioritized serine hydrolase targets of AA692, related to Table 2, Table S5. Visual MIC for Mtb::ribo-TesA, related to Table 2.

Protein name	Rv identifier	Function or annotation	Prioritized targetsa					Identified as a target of
			pH 6.6	pH 5.0	In vitro essential	In vivo essential6	Active under hypoxia	EZ12010	Lalistat10	CyC1711	THL12
AmiB2	Rv1263	putative amidase	●	●			●7,8			●
Rv1730c	Rv1730c	possible penicillin-binding protein	●		●1				●	●
LipC	Rv0220	esterase	●	●
Rv3591c	Rv3591c	probable hydrolase	●	●		●	●7
FbpA	Rv3804c	mycolyltransferase	●	●	●1,5	●	●7	●		●
Rv3802c	Rv3802c	lipase	●		●1,2,3,4	●	●8
FpbB	Rv1886c	mycolyltransferase	●	●			●7	●
Rv2627c	Rv2627c	alpha-beta hydrolase	●	●
TesA	Rv2928	thioesterase	●	●	●5	●	●7,8,9	●		●	●
LipM	Rv2284	probable hydrolase	●	●			●7,8,9	●	●	●	●
Cut2	Rv2301	probable carboxylesterase	●
LipE	Rv3775	probable lipase		●						●
LipG	Rv0646c	probable lipase		●		●
LipW	Rv0217c	probable esterase		●
Rv2854	Rv2854	probable lipase		●
LipN	Rv2970c	carboxylic ester hydrolase		●			●7,8		●	●
LipD	Rv1923	probable lipase		●
BpoC	Rv0554	putative non-heme bromoperoxidase		●						●

¹Griffin et al. (2011), ²Sassetti et al. (2003), ³DeJesus et al. (2017), ⁴Xu et al. (2017), ⁵FLUTE (orca2.tamu.edu/U19/), ⁶Zhang et al. (2013), ⁷Ortega et al. (2016), ⁸Tallman et al. (2016), ⁹Ravindran et al. (2014), ¹⁰Lehmann et al. (2016), ¹¹Nguyen et al. (2017), ¹²Ravindran et al. (2014).

^aListed in order of decreasing Δ(% inhibition), AA692-AA702 (see Table S4).