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. 2022 Feb 7;14:313–320. doi: 10.1016/j.bioactmat.2022.01.046

Fig. 2.

Fig. 2

PB-CRISPR enables robust knockout of protein-coding genes expressed in hPSCs.

A. Diagram of PB-CRIPSR plasmid design. B. Diagram of gRNA design to target THY1 gene. UTR: untranslated region. CDS: coding sequence. C. Flow cytometry data of THY1 knockout with either transient DNA delivery or PB-CRISPR insertion. D. Immunostaining images of IMR90C4 PB-CRISPR THY1 KO cells against CAS9. Scale bar: 100 μm. E. Diagram of gRNA targeting CTNNB1 gene. F. Immunostaining images of WT IMR90C4 cells or IMR90C4 PB-CRISPR CTNNB1KO cells against CAS9. Scale bar: 100 μm. G-H. Flow cytometry data of CTNNB1 knockout with either transient DNA delivery or PB-CRISPR insertion.