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. 2022 Feb 7;14:313–320. doi: 10.1016/j.bioactmat.2022.01.046

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — PB-CRISPR enables robust knockout of non-protein-coding genes that are not expressed in hPSCs. A. Diagram of gRNA design to target BANCR long non-coding RNA and primer design for knockout genotyping. B. Gel images of PCR products for BANCR knockout genotyping with single cell derived colonies. C. Genotype of the BANCR knockout OCT4-GFP H1 clone 1 cells. D. Dynamic BANCR expression during hPSC-CM differentiation. E. Relative expression of BANCR and NKX2.5 in D30 CMs derived from WT or PB-CRISPR BANCR KO OCT4-GFP H1 cells.