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. 2022 Mar 15;13:842340. doi: 10.3389/fimmu.2022.842340

Figure 4.

Figure 4

B cell specific RhoA deletion leads to developmental block at the pro B cell stage. (A) Flow cytometric analysis of freshly isolated cells from bone marrow (BM) of RhoAfl/fl and RhoAfl/fl Mb1+/hCre mice for surface expression of the indicated markers. Data are representative of 6 mice. Right: absolute cell numbers of B220+, pro-B cells (B220+CD43+), and pre-B cells (B220+CD43-) in the BM. (B) Flow cytometric analysis of cells isolated from spleen of RhoAfl/fl and RhoAfl/fl Mb1+/hCre mice. Right: absolute cell numbers of splenic B cells, immature (IgMhiIgD.), mature (IgMlowIgD+) MZ: marginal zone (CD21hiCD23-), FO: follicular (CD21lowCD23+). N=5 mice, statistical significance was calculated using two-tailed unpaired t-test. *p<0.05, **p<0.01, ***p< 0,001, ns, not significant.