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. 2022 Mar 15;10:827987. doi: 10.3389/fbioe.2022.827987

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Copyright © 2022 Wanner, Barnhart, Apostolakis, Zlojutro and Asosingh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating tree for lung cell phenotyping. The gating hierarchy for cell populations from the lung is shown. The expression or absence of defining markers are presented under each cell type. From the CD45⁺ cells: PACs, alveolar macrophages, interstitial macrophages, neutrophils and eosinophils are identified. CD45⁻cells are further divided into CD45-/Sca-1- and CD45-/Sca-1+ groupings. From the CD45-/Sca-1- group: myofibroblasts, pericytes, and epithelial goblet cells are identified. From the CD45-/Sca-1+ group: lymphatic endothelial cells (ECs), alveolar ECs, and blood vessel ECs are identified.