TABLE 1.
Antibodies used in spectral flow cytometry panel.
| Name | Alternative name | Clone | Fluorochrome | Source | Catalog number | Dilution by titration | Dilution by SC vs. MC |
|---|---|---|---|---|---|---|---|
| VWF | Von Willebrand Factor | Polyclonal | AF647 | Dako/Novus | A0082/336-0005 | 1/400 | 1/300 |
| CD90.2 | Thy-1.2 | 53–2.1 | BV421 | BioLegend | 140327 | 1/800 | 1/800 |
| α-SMA | ACTA2 | 1A4 | APC | Biotium | BNCA0665-250 | 1/800 | 1/800 |
| MUC2 | Mucin 2 | 996/1 | PE | Novus | NB120-11197PE | 1/200 | 1/75 |
| Ly-6G | Lymphocyte antigen 6 complex | 1A8 | AF532 | Novus | NBP2-53131AF532 | 1/800 | 1/800 |
| Siglec-F | Siglec-5 | E50-2440 | BV750 | BD | 747316 | 1/400 | 1/400 |
| CD11c | ITGAX | N418 | BB700 | BD | 745852 | 1/400 | 1/400 |
| CD24 | Heat stable antigen | M1/69 | BUV737 | BD | 612832 | 1/400 | 1/100 |
| CD11b | ITGAM | M1/70 | BV480 | BD | 566149 | 1/400 | 1/400 |
| Collagen-1 | Collagen type 1α | Polyclonal | FITC | Rockland | 600-402-103 | 1/200 | 1/100 |
| CD146 | S-endo 1 | ME-9F1 | BUV563 | BD | 741443 | 1/400 | 1/400 |
| PDGFRβ | CD140b | REA363 | APC-Vio770 | Miltenyi | 130-105-119 | 1/200 | 1/200 |
| EPCAM | CD326 | G8.8 | BV605 | BioLegend | 118227 | 1/200 | 1/200 |
| C-kit | CD117 | 2B8 | BV785 | BioLegend | 105841 | 1/400 | 1/400 |
| VEGFR2 | FLK-1; CD309 | Avas12a1 | BUV661 | BD | 741571 | 1/200 | 1/200 |
| MHC II | M5/114 | M5/114.15.2 | BUV805 | BD | 748844 | 1/200 | 1/150 |
| CD64 | Fcgr1 | X54-5/7.1 | BV650 | BD | 740622 | 1/200 | 1/200 |
| CD45 | Leukocyte common antigen | 30-F11 | BV570 | BioLegend | 103136 | 1/400 | 1/400 |
| Sca-1 | Ly-6a/e | D7 | BUV395 | BD | 563990 | 1/400 | 1/300 |
| Viability Dye | N/A | N/A | Live/Dead Red | Thermo Fisher | L23102 | 1/16000 | 1/16000 |