Figure 3.

GSK-J4 inhibits cytokine expression in macrophages. (A) The expression of cytokines in the culture media of BMDMs. (B–E) BMDMs were treated with GSK-J4 for 48 hours, the RNA levels of TNFα, IL12b, IL1β and IL10 were determined by qRT-PCR. (F–I) THP-1 cells were firstly differentiated with PMA for 72 hours and followed by treatment with GSK-J4 for 48 hours, the RNA levels of IL6, TNFα, IL1β and IL12b were measured by qRT-PCR. p < 0.05 (two-tailed Student’s T-test) is indicated by * for comparison with LPS+GSK-J4 vs. LPS, # for comparison with NC vs. LPS, error bars indicate SD.