Table 2.
Theoretical and measured molar mass of RCPhC1 obtained by MALDI-TOF. The theoretical molar mass of RCPhC1 is calculated from the sequence of its amino acids (https://web.expasy.org/protparam/).
Theoretical MM [Da] | Measured MM [Da] | Number of modified primary amines [n] | |
---|---|---|---|
Unmodified RCPhC1 | 51,185 | 51,188 | 0 |
RCPhC1-MA 0.5 EQ | 52,205 | 52,280 | 16 |
RCPhC1-MA 1 EQ | 53,429 | 53,380 | 32 |