Table 4.
Overview of the obtained data for RCPhC1-MA 0.5 and 1 EQ: Determination of the molar mass using MALDI-TOF and determination of the degree of substitutions (DS) using MALDI-TOF, 1H NMR spectroscopy (based on the known amino acid composition), OPA assay and shotgun proteomic analysis.
| MM obtained by MALDI-TOF [kDa] | DS obtained by MALDI-TOF [%] | DS obtained via 1H NMR (based on known AA composition) [%] | DS obtained via OPA [%] | DS obtained by shotgun analysis [%] | |
|---|---|---|---|---|---|
| RCPhC1-MA 0.5 EQ | 52.28 | 50.0 | 53.7 | 51.9 ± 1.6 | 66.2 |
| RCPhC1-MA 1 EQ | 53.38 | 97.0 | 93.3 | 99.2 ± 0.2 | 81.7 |