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The numbers of MA-modified lysines identified by LC-MS/MS with trypsin and Gluc digestion. The MA sites are identified on COL1α1 and COL1α2 from Bos taurus. Based on the AA sequences from the Swiss-Prot database, the total number of lysines for COL1α1 and COL1α2 were 38 and 31, respectively.
| Trypsin digestion | GluC digestion | ||
|---|---|---|---|
| COL-MA BS 0.5 EQ | COL1α1 | 30 | 17 |
| COL1α2 | 23 | 16 | |
| COL-MA BS 1 EQ | COL1α1 | 26 | 19 |
| COL1α2 | 21 | 15 |
