Table 8.

Determination of the degree of substitution (DS) of COL-MA BS 0.5 and 1 EQ using ¹H NMR spectroscopy (based on the amino acid sequence from the Swiss-Prot database, and the analyzed amino acid composition), OPA assay and shotgun proteomic analysis.

	DS obtained via 1H NMR (based on database Swiss-Prot) [%]	DS obtained via 1H NMR (based on known AA composition) [%]	DS obtained via OPA [%]	DS obtained via shotgun analysis [%]
COL-MA BS 0.5 EQ	76.6	75.9	74.1	74.9
COL-MA BS 1 EQ	103.7	99.6	95.8	80.4