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. 2022 Feb 21;13(5):1398–1409. doi: 10.7150/jca.58053

Figure 3.

Figure 3

miR-184-5p regulates invasion and apoptosis of A-498 and 786-O cells. (A-B) Up-regulation of miR-184-5p could significantly reduce cells invasion capacity. The cell numbers markedly increased after transfection with of miR-184-5p inhibitor (P<0.01) and dropped after transfection with of miR-184-5p mimics (P<0.01), compared with the negative control group. (C-D) After transfection with miR-184-5p inhibitor in A-498 and 786-O cells, we found significantly decreased apoptosis cells compared with negative control cell groups measured by propidium iodide (PI) and FITC ‐ Annexin V fluorescence. Annexin V-FITC (-) PI (-), E3, stands for normal cells; Annexin V-FITC (+) PI (-), E4, stands for early apoptotic cells; Annexin V-FITC (+) PI (+), E2, stands for late apoptotic cells; Annexin V-FITC (-) PI (+), E1, stands for mechanical necrotic cells. Both early and late apoptotic cell (E4 and E2) were counted and measured as apoptotic cells. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.