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. 2022 Mar 14;13(5):1664–1678. doi: 10.7150/jca.57691

Figure 3.

Figure 3

Knockdown of circASPM suppressed the proliferation of GSCs in vitro. a: The expression of circASPM in GSC17 and GSC18 after transfection of circASPM-KD1, circASPM-KD2 or negative control as measured by qPCR. b, c: CircASPM knockdown significantly reduced the proliferation of GSC17 and GSC18 in MTS assays. d, e, f: CircASPM knockdown can significantly increase the proliferative capacity of GSC17 and GSC18 as measured by Edu assays. Scale bar = 50μm. g, j, h, i: Neurospheres formation assays and extreme limiting dilution assays showed that the tumor formation rates decreased after circASPM knockdown in GSC17 and GSC18. Scale bar = 50μm. All data are shown as the mean ± SD (three independent experiments). *P < 0.05; **P < 0.01; ***P < 0.001.