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. 2022 Mar 14;13(5):1664–1678. doi: 10.7150/jca.57691

Figure 5.

Figure 5

CircAPSM regulate E2F1 expression via miR-130b-3p sponging. a: Schematic diagram of the putative miR-130b-3p binding site in the 3′-UTR of E2F1. b, c, d, e: The luciferase reporter assays showed that miR-130b-3p mimic or inhibitor altered the luciferase promoter activities of E2F1. f, g, h, i, j, k: qPCR and western blotting showed the expression of E2F1 in GSCs after miR-130b-3p mimic or inhibitor treatment. l, m, n: The decreased or increased expression of E2F1 in GSCs induced by cARF1 knockdown or overexpression was reversed by miR-130b-3p inhibitor or mimic treatment, as determined by western blotting and qPCR. All data are shown as the mean ± SD (three independent experiments). *P < 0.05; **P < 0.01; ***P < 0.001.