FIGURE 8.

Recapitulation of Lrap. We previously identified a novel transcript, subsequently annotated Lrap, as a potential mediator of alcohol consumption that is expressed in rat brain (Saba et al., 2015; Saba et al., 2020). The structure identified previously is shown in green (Lrap), the de novo transcript identified in the detected above background (DABG) transcriptome is shown in blue (MSTRG.6250.1), and existing Ensembl annotation is shown in red (ENSRNOT00000077521). The transcript identified here as MSTRG.6250.1 (annotated by StringTie) closely matches our previous annotation of Lrap. Additionally, the RNA sequencing reads on the negative strand (black plot) support the present of this transcript in our dataset. The RNA sequencing reads represent a 10% randomly sampled subset from the HXB/BXH recombinant inbred rat panel RNA sequencing data in brain. This image was generated using the UCSC Genome Browser (Kent et al., 2002).