Table 1.
LAMP-based detection of various pathogens in wheat.
| Pathogen | Disease | Target gene | Visualization technique | References |
|---|---|---|---|---|
| Wheat dwarf virus (WDV) | Wheat dwarf | Coat protein | Gel electrophoresis, real-time monitoring of amplification curves, SYBRGreen I dye | Trzmiel and Hasiów-Jaroszewska, 2020 |
| Wheat streak mosaic virus (WSMV) | Wheat streak mosaic | Poly-protein coding gene | Electrophoresis in agarose gel | Lee et al., 2015 |
| Wheat yellow mosaic virus (WYMV) | Wheat yellow mosaic | Coat protein | Turbidity observation and electrophoresis | Zhang et al., 2011 |
| Pyricularia oryzae Triticum lineage | Wheat blast | PoT2 and MoT3 loci | Real-time fluorescence and Genie II system | Yasuhara-Bell et al., 2018 |
| Puccinia triticina (synonym: P. recondita f. sp. tritici) | Leaf rust | PtRA68 specific marker | Hydroxy naphthol Blue (HNB) visualizing indicator; electrophoresis in agarose gel | Manjunatha et al., 2018 |
| Puccinia striiformis f. sp. tritici | Wheat stripe (or yellow) rust | β-tubulin gene | SYBR Green I and electrophoresis in agarose gel | Huang et al., 2011 |
| Ketopantoate reductase coding sequence | HNB dye and ethidium bromide, electrophoresis | Aggarwal et al., 2017 | ||
| Tilletia caries, T. controversa, and T. laevis | Common bunt, dwarf bunt and smooth-spored wheat bunt | IGS 2 rDNA | Real-time monitoring with melting curves, electrophoresis, and direct fluorescence | Pieczul et al., 2018 |
| Ustilago tritici | Loose smut of wheat | Large ribosomal unit and ITS region | SYBR Green I | Yan et al., 2019 |
| Fusarium asiaticum | Fusarium head blight | CYP51C gene | Hydroxy naphthol Blue (HNB) visualizing indicator | Xu et al., 2017 |
| Fusarium graminearum | Fusarium head blight | 218-bp region from a partial sequence of F. graminearum chromosome 1 | Hydroxy naphthol Blue (HNB) visualizing indicator; electrophoresis in agarose gel | Gupta et al., 2020 |
| gaoA gene (galactose oxidase precursor) | Real-time calcein fluorescence; electrophoresis in agarose gel | Niessen and Vogel, 2010 | ||
| Fusarium species | Deoxynivalenol (DON), nivalenol (NIV) and T2-Toxin | tri5 gene (trichodiene synthase) and tri6 (biosynthesis of trichothecenes) | Real-time turbidimeter amplification curves | Denschlag et al., 2014 |
| Zymoseptoria tritici (fungicide resistance) | Septoria tritici blotch | MgCYP51 and MgMFS1 genes | Gel electrophoresis | King et al., 2016 |