Table 2.
Isothermal-based detection of various pathogens in wheat.
| Isothermal-based technique | Pathogen | Disease | Target gene | References |
|---|---|---|---|---|
| RPA | Bipolaris sorokiniana | Root rot and spot blotch | Calmodulin (cal) | Zhao et al., 2021 |
| RPA | Wheat dwarf virus (WDV) | Wheat dwarf | Polymorphic 12 nucleotides motif (nt 1,433–1,444) | Glais and Jacquot, 2015 |
| RT-RPA | Barley yellow dwarf virus (BYDV) | Yellow dwarf of wheat | Coat protein (CP) gene | Kim et al., 2020 |
| RCA | Fusarium graminearum species complex (FGSC) | Fusarium Head Blight (FHB) | Elongation factor 1-α (EF-1α) | Davari et al., 2012 |
| RT-HDA | High plains virus (HPV) | High plains of wheat | Nucleoprotein gene | Arif et al., 2014 |
RPA, recombinase polymerase amplification; RT-RPA, reverse transcription RPA; RCA, rolling-circle amplification; and RT-HDA, reverse transcription helicase-dependent amplification.