Table 3.
Recent LAMP-based approaches for detection of pathogens in other plants.
| LAMP-based approach | Pathogen | Disease | Target sequence | References |
|---|---|---|---|---|
| Multiplex RT-LAMP | Banana bunchy top virus (BBTV), banana streak viruses (BSVs), cucumber mosaic virus (CMV) | Banana bunchy top, banana streak, cucumber mosaic | Conserved regions of coat protein sequence | Zhang et al., 2018 |
| Portable LAMP. Genie II instrument | Neofabraea perennans | Bull’s eye rot (BER) in apple and pear | β-tubulin gene | Enicks et al., 2020 |
| LAMP-Coupled CRISPR-Cas12a module | Tomato yellow leaf curl virus (TYLCV) and Tomato leaf curl New Delhi virus (ToLCNDV) | Tomato yellow leaf curl and Tomato leaf curl New Delhi | Coat protein gene (CP) | Mahas et al., 2021 |
| Real-time colorimetric LAMP | Xanthomonas gardneri | Bacterial spot (BS) of tomato and pepper | hrpB gene | Stehlíková et al., 2020 |
| Probe-based real-time LAMP | L. acicola, D. pini and D. septosporum | Spot needle blight (BSNB) and Dothistroma needle blight (DNB) | Elongation factor (EF1- α) and beta-tubulin (β-tub2) | Aglietti et al., 2021 |
| FRET-based probe qLAMP | Erysiphe necator | Grape powdery mildew | ITS region | Thiessen et al., 2018 |
| LAMP-based Turn-on Fluorescent Paper (ToFP) | Rosellinia necatrix | White root rot (WRR) | Template candidates from regions in strain W97, scaffold, contig 1 sequence | Lee et al., 2020 |
| Microneedle-smartphone-based LAMP and RT-LAMP | Phytophthora infestans and Tomato spotted wilt virus (TSWV) | Late blight on potato and tomato, and tomato spotted wilt | ITS sequence in P. infestans and N gene of TSWV. | Paul et al., 2021 |
| Cas12a PAM-free LAMP (Cas-PfLAMP) | Xanthomonas oryzae pv. Oryzae, rice stripe virus (RSV), and rice black-streaked dwarf virus (RBSDV) | Rice bacterial leaf blight, rice stripe, rice black-straked dwarf | PilV gene from X. oryzae pv. Oryzae, RSV SD-JN2 RNA4 segment, RBSDV N89 P1 gene. | Zhu et al., 2022 |