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. 2022 Mar 15;13:858806. doi: 10.3389/fmicb.2022.858806

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Copyright © 2022 Li, Xiao, Yang, Yao, Li, Zheng, Guo, Wang, Chen, Guo, Wang and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical specificity of RPA-CRISPR/Cas12a assay for MP detection. The RPA-CRISPR/Cas12a assay is conducted using genomic DNA extracted from 19 pathogens. Tube 1-5, clinical isolates of MP; Tube 6-19, Klebsiella pneumonia ATCC 10031, Klebsiella pneumonia ATCC 700603, Klebsiella pneumonia isolated strain, Haemophilus influenzae isolated strain, Acinetobacter baumannii ATCC 19606, Acinetobacter baumannii isolated strain, Streptococcus pneumoniae isolated strain, Staphylococcus aureus isolated strain, Staphylococcus aureus isolated strain, Pseudomonas aeruginosa isolated strain, Group B Streptococcus isolated strain, isolated strain of carbapenem-resistant Enterobacter sp., Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212; PC, positive control; NC, negative control.