TABLE 4.
Comparison of the current approach with other isothermal amplification methods for MP detection.
| Approach | No. of primers | Temperature (°C) |
Reaction time (min) |
Sensitivity (copies per reaction) |
Clinical samplea | References |
| RPA-CRISPR/Cas12a assay | 2 | 37/39 | 27-32 | 2 | 201 respiratory specimens (128 swab samples, 40 BALF samples and 33 sputum samples) | This study |
| RAA assay (fluorescent probe-based method) | 2 | 39 | 15-30 | 2.23 | 311 respiratory specimens (213 BALF samples, 90 sputum samples and 8 swab samples) | Xue et al., 2020 |
| MCDA-LFB assay | 10 | 65 | 32 | ∼50 (50 fg) |
197 swab specimens | Wang et al., 2019c |
| LAMP-LFB assay | 6 | 65 | 62 | ∼600 (600 fg) |
209 swab specimens | Wang et al., 2019b |
aBALF, bronchoalveolar lavage fluid.