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. 2022 Mar 6;12(6):2658–2673. doi: 10.7150/thno.64244

Figure 3.

Figure 3

In vitro antibacterial activity of GOX-HMSN-AZM. (A) Planktonic cultivation turbidity (S. aureus) with 0, 3.91, 7.81, 15.63, 31.25, 62.50 μg/mL of HMSN, HMSN-AZM, GOX-HMSN and GOX-HMSN-AZM after standard incubation for 24 h. (B) The corresponding survival rate of S. aureus incubated with different concentrations of HMSN, HMSN-AZM, GOX-HMSN and GOX-HMSN-AZM after 24 h. (C) Live/dead staining assay of S. aureus incubated with PBS, HMSN, HMSN-AZM, GOX-HMSN and GOX-HMSN-AZM by confocal fluorescence microscopy. Live bacteria were stained with SYTO9 (green) and dead bacteria were stained with PI (red). Scale bar = 20 μm. (D) TEM micrographs of S. aureus incubated with PBS, HMSN, HMSN-AZM, GOX-HMSN and GOX-HMSN-AZM. Up bar = 0.1 μm, bottom bar = 0.5 μm.