Table 1.

Bacterial strains and plasmids used in this study.

Strains/plasmids	Characteristics/DNA sequences	References
E. coli
E. coli Top10	F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG	Invitrogen
ClearColi BL21(DE3)	F- ompT hsdSB (rB- mB-) gal dcm lon λ (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) msbA148 ∆gutQ ∆kdsD ∆lpxL ∆lpxM ∆pagP ∆lpxP ∆eptA	Lucigen
Plasmids
pUC57 n protein	ApR, ColE1 origin, cloning vector containing XhoI/BamHI fragment gene n protein	Biomatik
pUC57 rbd 5' end	ApR, ColE1 origin, cloning vector containing XhaI/NotI fragment gene rbd	Biomatik
pUC57 rbd 3' end	ApR, ColE1 origin, cloning vector containing XhoI/BamHI fragment gene rbd	Biomatik
pET-14b phaC	pET-14b containing phaC DNA fragment; ApR; T7 promoter	57
pET-14b phaC-rbd	pET-14b containing XhoI/BamHI fragment gene rbd fused to the 3' end of phaC	58
pET-14b rbd-phaC-n protein	pET-14b containing XbaI/NotI fragment gene rbd and XhoI/BamHI fragment gene n protein fused to the 5' end 3' end of phaC respectively	58
pMCS69	CmR, pBBR1 MCS derivative containing phaA and phaB from Cupriavidus necator, colinear to lac promoter	59

Ap^R, ampicillin resistance; Cm^R, chloramphenicol resistance.