FIGURE 1.

Experimental setup of the cerebellar explant cultures. Cerebella of newborn tenascin-C and tenascin-R knockout and wildtype mice pups were cut into 250–300 µm thick sagittal sections and cultivated in slice culture medium. Demyelination was induced by administration of 0.5 mg/ml lysolecithin to the medium for 16-18 h. For remyelination analysis, explants were cultivated for further 14 days. Untreated control explants (C) were kept for the whole duration of the experiment.