FIGURE 3.

(A,B) Tnc inhibits remyelination in cerebellar slice cultures. (A,A′,B,B′) Exemplary cerebellar explants cultivated in the absence (A,B) or presence (A′,B′) of Tnc. Slices were stained for NF-200 and MBP. Tnc-treated remyelinated cultures displayed myelin membranes that did not wrap the axons throughout the whole cerebellar slice. Scale bar: 20 μm. (B) Evaluation of myelination index, defined as the proportion of colocalized area among the areas of complete neurofilament staining. These results indicate an impaired remyelination in Tnc-treated cultures. Data are presented as mean ± SEM, and statistical significance was assessed using an unpaired two-tailed Student’s t-test for each group (remyelinated and control). Six independent (N = 6) experiments were performed, and three explants (n = 3) were analyzed per experiment for each condition.